NMR allows one to study ligand−protein interactions, either by exploiting effects of binding on the linewidths (1/T 2 or 1/T 1ρ ) of prospective drugs, or by observing effects on the lifetimes of long-lived states (LLSs). The latter method requires very low protein−ligand ratios, thus saving on costly proteins. Screening inhibitors of urokinase-type plasminogen activator (uPA)
which is a prototypical target of cancer research, with a concentration of only  10 μM, allowed us to determine a dissociation constant K D = 180 ± 20 nM for a strong inhibitor. The more cumbersome surface plasmon resonance method gave K D = 157 ± 39 nM. 

C.V. 

Ecole Montessori, La Haye, Pays-Bas, 1957-62.
Ecole Internationale, Genève, Suisse, 1963-70.
Eidgenössische Technische Hochschule Zürich (ETH), Suisse, 1970-74.
University of Oxford, England, 1975-77, Doctoral thesis (D.Phil., Ray Freeman.)
University of California at San Diego, La Jolla, 1978, post-doctoral  fellowship.
Research Staff, MIT (1979-1980).
Oberassistent, Eidgenössische Technische Hochschule Zürich (ETHZ),1980-85.
Professeur, University of Lausanne,1985-2001.
Professeur, Florida State University, Tallahassee, Florida, USA,1994 –1996.
Professeur, Swiss Federal Institute of Technology, Lausanne, Suisse, 2001- 2016.
Professeur 1996-2016, émérite 2016-2023, Ecole Normale Supérieure. 

Publications récentes 

Polychromatic Excitation of Delocalized Long-Lived Proton Spin States in Aliphatic Chains. A. Sonnefeld, G. Bodenhausen, and K. Sheberstov, Phys. Rev. Letters, 129, 183203, 1-6 (2022); DOI: 10.1103/PhysRevLett.129.183203

Drug Screening of Achiral Molecules by Long-Lived States in NMR.A.
Sonnefeld, A. Razanahoera, P. Pelupessy, G. Bodenhausen, and K.
Sheberstov. Science Advances, 8, eade2113 (2022); DOI:
10.1126/sciadv.ade2113